The type-IV secretion system (T4SS) is a machinery able to transfer DNA and proteins between bacteria and in certain cases also to eukaryotic cells.

Promotor: Johan Kuiper, Co-promotor: Saskia C.A. de Jager

The investigations described in this thesis lay out strategies aimed at advancing antibiotic research and development. The examples presented revolve around two main approaches: understanding drug-target interactions and target identification.

Promotor: Roeland M.H. Merks

The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

In previous studies at our laboratory it was demonstrated that drug exposure of HepG2 cells can lead to an altered TNFα-induced NF-κB oscillatory phenotype, concurrent with a synergistically increased sensitivity for TNFα-induced apoptosis.

In this thesis, bioorthogonal chemistry is combined with correlative light-electron microscopy to selectively label and study pathogenic intracellular bacteria within the host immune cell.

Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

Image-Pro Plus, ImageJ, Cell Profiler, R, Knime en NIS-elements

Nierfalen is een serieus probleem omdat nieren zichzelf niet kunnen regenereren na ernstige beschadiging. Een mogelijke oplossing bestaat uit het kunstmatig laten groeien van gezond nierweefsel. Daarvoor moeten wetenschappers eerst de ontwikkeling begrijpen tijdens de allereerste fase, in de foetus.…

Onderzoeken en ontwerpen zijn twee vaardigheden die steeds belangrijker worden in de vakken O&O (onderzoeken en ontwerpen) en NLT (natuur, leven en technologie). Uit onderzoek van Tessa Vossen (ICLON en Faculteit Wiskunde en Natuurwetenschappen) blijkt dat docenten en leerlingen de relevantie van onderzoeken…

The function of TLRs in innate immunity has aroused worldwide attention soon after its discovery. Because of the broad functions of TLR2 in innate immunity, the drive for the development of TLR2-targeted vaccines or therapeutic treatments has accelerated in the last decades.

The applications of studying the early development of insects range from agriculture to material science.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

Promotores: A.H. Meijer, H.P. Spaink

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

Nanodiscs are small, disc-shaped structures designed to mimic biological cell membranes, composed of a lipid bilayer stabilized by scaffold proteins. Membrane proteins play crucial roles in essential processes such as nutrient and waste transport, molecular recognition, and cell signalling, making them…

As a virtually inexhaustible source, solar energy plays a major role in future global energy scenarios.

Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea

This thesis describes the development of a variety of mannosylated conjugates. Antigen presenting cells bear mannoside recognizing receptors that actively transport antigen into the cell. This thesis exploits this feature for the development of improved vaccines.

Viral diseases constitute one of the major challenges in modern medicine. Membrane-targeting have broad-spectrum potential and could distinguish viral membranes from the host cell plasma membrane by the difference in membrane curvature.

Promotores: C.A.M.J.J. van den Hondel, V. Meyer, Co-promotor: A.F.J. Ram

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

Promotor: H.J.M. de Groot

In 2024 hebben medewerkers en studenten van de Universiteit Leiden veel mooie prijzen gewonnen en belangrijke onderzoekssubsidies binnengehaald.

Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research (LACDR)

In this thesis, we will utilize embryonic zebrafish tumour models to understand the interaction between engrafted human cancer cells and macrophages from the host, test drug administration modalities and anti-cancer efficacies of newly-developed PDT and PACT compounds, and test a light-triggered liposomal…

Promotie

Promotie

Hoe worden nachtruimtes verbeeld, geproduceerd, ervaren en verteld door migrantengemeenschappen in Europa? Dit onderzoeksproject onderzoekt deze vraag in acht Europese steden: Aarhus, Amsterdam, Berlijn, Cork, Galway, Lissabon, Londen en Rotterdam.

Maandelijks interviewen we een student of medewerker van de Universiteit Leiden over hun werk of studie in relatie tot duurzaamheid.

The use of increasingly large and diverse datasets to guide urban climate action has implications for how, and by whom, local governments are held accountable.

Leidse expertise over Noord-Amerika strekt van slavernij, migratie en emancipatie, geschiedenis, internationale betrekkingen en mensenrechten tot etniciteit, identiteit, politiek en milieu.

Pericytes, the mural cells of blood microvessels, are important regulators of vascular morphogenesis and function that have been postulated to mechanically control microvascular diameter through as yet unknown mechanisms.

The research described in this Thesis was aimed at designing and synthesizing nature-inspired compounds as part of TB vaccine discovery.

In this thesis, time-lapse fluorescent microscopy plays a pivotal role in investigating functional materials within living cells as well as the migratory behaviour of neural progenitor cells.

In this thesis, we focus on novel proteins/mechanisms that regulate integrin-adhesion mediated mechano-transduction.

The aim of this thesis is to explore the use of Bioorthogonal Antigens to study the cross-presentation pathway.

Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber

This thesis focused on the exploration of monolayer graphene as a membrane in direct methanol fuel cell (DMFC) applications, with improvements achieved through plasma exposure or damage- free chemical functionalization.

This thesis focuses on the role of chemokine receptors CXCR3 and CCR2 in the inflammatory process and infection control using the zebrafish model.

Promotie

Promotie

Perspectieven zouden het fundament van een toekomstbestendig curriculum in alle onderwijssectoren moeten zijn

Didactiek

Met een perspectiefgerichte benadering van onderwijs worden kennis, vaardigheden en houding van leerlingen met elkaar verbonden.

Aan het LUCL bekijken we bijna alle aspecten van een grote hoeveelheid Afrikaanse talen. Van fonologie tot antropologische taalkunde, van theoretische syntaxis tot straattaal: we onderzoeken het allemaal.