Promotor: J.G.E.M. Fraaije, Co-Promotor: A. Kros

Lipid signaling is an essential biological event/process in a plethora of pathophysiological conditions. The underlying idea of this thesis is that many of the roles and the complex interplay of the individual signaling lipids in inflammatory processes and related conditions in health and disease is…

Glycoside hydrolases (glycosidases/GHs) are widely abundant enzymes in all kingdoms of life and are important biocatalysts that catalyze the hydrolysis of glycosidic linkages in oligo/polysaccharides, glycoproteins and glycolipids with tremendous efficiency

Marine sponges are important members of reef ecosystems, as they play ecological roles that are essential for the health of the reef.

The recently introduced nature-originated deep eutectic solvents, so-called natural deep eutectic solvents (NADES) are considered as truly green solvents, which composed of natural ingredients found abundantly in organisms.

This Thesis focuses on the design and synthesis of ADP-ribosylated compounds that can be applied in biological studies.

Glycosidases are essential in fundamental biological processes and are responsible for the degradation of most (oligo)saccharides, glycolipids and glycoproteins.

Promotor: Prof.dr. T. Hankemeier, Co-promotor: Theo Reijmers

Carbohydrates, alongside proteins and nucleic acids, constitute a crucial and versatile family of biomolecules present in all life forms. They manifest as monosaccharides, oligosaccharides, and polysaccharides, covalently bonded to proteins and fats. Carbohydrates are integral to plant and arthropod…

This work has described synthetic strategies towards well-defined structures resembling capsular polysaccharide (CPS) fragments, CPS mimics, teichoic acid (TA) fragments as well as a third-generation ring-closing tandem metathesis (RCM) linker to better exploit the potential of automated synthesis.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

We have studied shape with a particular focus on the zebrafish model system. The shape is an essential appearance of the phenotype of a biological specimen and it can be used to read out a current state or response or to study gene expression.

In this thesis an activity-based probe was discovered that could visualize the activity of PLAATs. With an optimized gel-based ABPP assay in hand, screening of a compound library led to the discovery of alpha-ketoamides as a hit for PLAAT3.

Neurodegenerative diseases, including Parkinson’s disease (PD), are increasing in prevalence due to the aging population. Despite extensive study, these diseases are still not fully understood and the lack of personalised treatment options that can target the cause of the diseases, rather than the symptoms,…

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Promotor: J. van der Greef; Co-promotor: E. van Wijk, M. Wang

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-Promotor: J.D.C. Codee

This thesis focuses on the application of lipid-based nanomedicine in drug delivery, including small molecular antitumor drugs and biomacromolecules including mRNA, and evaluates their biological performance.

Within this thesis the central stage is taken by the discovery and investigation of transglycosylation of sterols. First, investigation focuses on the development of a method to accurately detect and quantify glucosylated metabolites in biological materials.

Transcutaneous vagus nerve stimulation (tVNS) was tested in chronic worriers. tVNS may reduce spontaneously occurring negative thought intrusions. After a worry induction, there was no longer an effect of tVNS. tVNS did not affect physiological responding to worrying.

De verklaring van natuurkundige Scott Waitukaitis voor de gillende en stuiterende hydrogelballetjes is door verschillende media overgenomen. Zo was Waitukaitis te zien op het RTL 4 journaal van maandag 24 juli.

Een overzicht van blogs, video's, podcasts, boeken, artikelen en activiteiten waarmee u zich verder kunt verdiepen in het koloniale en slavernijverleden.

Op een ogenschijnlijk gewone kubus verschijnt onder druk een patroon van hollingen en bollingen. Er is nu een methode ontwikkeld om zulke 3 dimensionale structuren te ontwerpen en maken met eenvoudige bouwstenen. Publicatie in Nature.

Kleinere computerchips, smallere cameraatjes; ze worden steeds moeilijker om te maken. Maar wat als de bouwstenen zichzelf opbouwen? Natuurkundige Daniela Kraft bedacht een methode om deeltjes aan te sturen in samenklonteren. Publicatie in ACS Nano.

Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov

Promotor: Prof.dr. E.J.J. Groenen, Co-Promotor: M. Huber

Because thin systems can deform along the thickness with relative ease, the interplay between surface mechanics and geometry plays a fundamental role in sculpting their three-dimensional shape.

The thesis focuses on synthesis strategies in oligosaccharide campaigns, and the influence of protecting groups.

This Thesis describes the design, synthesis and evaluation as glycoprocessing enzyme inhibitors of focused libraries of iminosugars.

Drug-target binding kinetics determine the time course of the central event in pharmacotherapy: Drug-target interaction.

Promotor: A. Doelman, Co-promotores: P.J.A. van Heijster; A. Zagaris

De tweejarige masteropleiding Physics wordt aangeboden door het Leids Instituut voor Onderzoek in de Natuurkunde. De opleiding is theoretisch en experimenteel. Er is ruime aandacht voor onderzoekstraining. Ook bieden we veel keuzemogelijkheden en op maat gemaakte, individuele studieprogramma’s.

Na het afronden van de bacheloropleiding volgen verreweg de meeste studenten de masteropleiding.

Promotie

Promotie

Waterdruppels zweven in een hete pan vanwege het zogenoemde Leidenfrost-effect. Nu hebben natuurkundigen een variatie ontdekt: het Elastische Leidenfrost-effect. Het verklaart waarom hydrogel balletjes op een hete plaat rondspringen en hoge piepgeluidjes maken. Publicatie in Nature Physics op 24 jul…

Hoe complexer het voorwerp, des te ingewikkelder het is om op te vouwen. Een ruimtevaartuig heeft al snel vele motortjes nodig om een instrument-arm in elkaar te vouwen en mensen hebben zelfs moeite met een wegenkaart. Natuurkundigen uit Leiden en Amsterdam hebben nu een structuur ontworpen die zichzelf…

Supervisor: P.J.J. Hooykaas

Promotor: Prof.dr. J.N. Kok, Co-Promotor: W.A. Kosters

We have studied the impact of particle shape anisotropy, multivalent interactions and flexibility on systems of micron-sized colloidal particles.

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

The progressive loss and degeneration of dopaminergic neurons is a major pathological hallmark of Parkinson's disease (PD). The onset and progression of PD can be triggered by multiple risk factors, for instance, genetic mutation, environmental exposure, and aging.

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

This thesis describes the synthesis and biological evaluation of TLR2/6, TLR4, TLR7/8 and TLR9 ligands, of which the activity can be conditionally controlled.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

Triple-negative breast cancer (TNBC) constitutes a small subtype (~15%) of breast cancer, but causes the majority of breast cancer-related deaths.

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

This thesis describes the development and optimization of the first molecular tools to study the enzyme PLA2G4E.

Promotor: Prof.dr. M. Ubbink