In het boek Uitdagend gedifferentieerd vakonderwijs van Janssen, Hulshof en Van Veen wordt een ‘generatieve toolkit’ beschreven voor het aanpassen van het onderwijs door leraren.

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

In dit project wordt onderzocht hoe de interactieprocessen verlopen binnen professionele leergemeenschappen en wat het effect hiervan is op differentiatie in de klas van vmbo-docenten.

Hoe kunnen docenten de gespreksvaardigheid in MVT van de individuele leerling in klassen van 30 verbeteren?

Motivatie en prestaties van scholieren lijken minder te worden tijdens de middelbare schoolperiode. Deze tendens willen we graag verminderen. Daarom is GUTS (Gedifferentieerd Uitdagen van Talent op School) bedacht. Deze interventie, die nu wordt uitgevoerd op één school, is een combinatie van een verhoogde…

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Mechanical interactions between cells and their environment play an important role in many biological processes.

Promotor: Prof.dr. J. Kuiper

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Welke relatie bestaat er tussen verschillende type PLGs en cognitieve differentiatie?

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

In this thesis, we have studied cell images from two types of cells, including pollen grains and the immune cells, neutrophils. These images are captured using a bright field microscope and a confocal microscope.

Traditional drug discovery approaches have been hampered by (in vitro) cell-culture models that poorly represent the situation in the human body.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Leerlingen verschillen van elkaar, daarom zit er veel differentiatie in ons onderwijssysteem. Denk aan vwo- en vmbo-scholen, maar ook niveaugroepjes in de basisschoolklas. Maar volgens bijzonder hoogleraar Eddie Denessen kan differentiatie ook de kansenongelijkheid juist vergroten. Oratie op 26 juni…

There is an urgent need for more physiologically relevant cell culture methods to guide compound selection in pre-clinical stages of the drug development pipeline.

This thesis focuses on two processes involved in fighting infections: metabolism and immune cell motility and navigation.

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

This thesis describes the design and synthesis of fragments of various cell wall carbohydrates of the Streptococcus species, including the branched Group B-specific antigen (GBC) of Group B Streptococcus, glycerol phosphate (GroP) modified group A carbohydrate (GAC), and the O-acetylated type 1 capsular…

Veel docenten in het voortgezet onderwijs houden rekening met verschillen tussen leerlingen. Uit het promotieonderzoek van Saskia Stollman (ICLON) blijkt dat zij hierbij ruimte om te experimenteren en ondersteuning van de school(leiding) nodig hebben. Promotie op 23 mei 2018.

CONCLUSION: This study demonstrates that human macrophages can be induced to exert direct anti-tumor activity against osteosarcoma cells. Our observation that the induction of macrophage anti-tumor activity by L-MTP-PE required IFN-gamma may be of relevance for the optimization of L-MTP-PE therapy in…

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

In dit onderzoek worden docenten gevolgd op een school waar een project loopt waarin leerlingen worden uitgedaagd hun talenten te ontwikkelen.

Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Promotores: Prof.dr. G.F.A. Kersten, W. Jiskoot, Co-promotor: J-P. Amorij

Synthetic hydrogels that mimic the natural extracellular matrix in the biophysical and biochemical cues it provides to cells are in high demand, however the cell phenotypes as they are observed in vivo in numerous cases have yet to be attained.

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

Promotor: C.A.J. Knibbe, Co-promotor: J.J. Boelens, R.G.M. Bredius

Promotor: Prof.dr. R. Verpoorte, Co-Promotores: N.R. Mustafa, A.E. Schulte

Light-Harvesting Complex II (LHCII) is responsible for light absorption and excitation energy transfer in plants and photosynthetic algae, while in high light it undergoes conformational changes by which it quenches excitations to prevent photodamage.

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

Veel docenten in het voortgezet onderwijs houden rekening met verschillen tussen leerlingen. Uit het promotieonderzoek van Saskia Stollman (ICLON) blijkt dat zij hierbij ruimte om te experimenteren en ondersteuning van de school(leiding) nodig hebben.

Promotor: A.H. Meijer, Co-promotor: B.E. Snaar-Jagalska

Carbohydrates are present on the surface of bacteria making them suitable antigen candidates for vaccine development. This thesis deals with the synthesis of two carbohydrate-based components; the capsular polisaccharide of S. aureus type 5 and teichoic acids from staphilococci and enterococci speci…

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

This thesis describes the antimicrobial discovery strategy developed in our group, the den Hertog Group at the Hubrecht Institute. It includes a cultivation-based screening approach for novel antimicrobial agents from the source of fungi, and a bacterial time-lapse imaging approach for antimicrobial…

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

Promotor: G.A. van der Marel, Co-promotor: J.D.C. Codée