Deze opleiding integreert genetica, moleculaire biologie en biotechnologie in een aantrekkelijk programma met uitstekende carrièreperspectieven.

Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.

A structural investigation of coiled coil peptides used as membrane fusogens, mimicing naturally occuring coiled-coil fusion proteins. Synthetic modifications have been made to alter lipid attachment, secondary structure and to insert photoactive azobenzene moieties for active control over coiled coil…

Promotor: Prof.dr. T. Schmidt, Co-Promotor: S.J.T. van Noort

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Promotor: Prof.dr. G.R. de Snoo

Promotor: P.M. Brakefield

Hoe kunnen we met behulp van moleculair gereedschap meer inzicht krijgen in de ziekteverwekkende eigenschappen van de Neisseria pili?

DNA carries various forms of information. Out of these forms of information the most well-known is classical genetic information.

The four possible segments A, T, C and G that link together to form DNA molecules, and with their ordering encode genetic information, are not only different in name, but also in their physical and chemical properties.

The applications of studying the early development of insects range from agriculture to material science.

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Freshwater is an important resource, but at a great risk of species decline due to habitat loss, pollution and over-exploitation, and invasive alien species.

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

What is the population size/structure and distribution of tiger and leopard inside the Bardia National Park (BNP) compared with the population outside? What is the movement and home range and conflict pattern in time and space of tigers and leopards inside the BNP compared with outside? What constitutes…

This thesis describes the genetic and prokaryotic diversity of giant barrel sponges.

Supervisor: P.J.J. Hooykaas Co-Supervisor: R. Offringa

The ongoing increase in antimicrobial resistance combined with the low discovery of novel antibiotics is a serious threat to our health care.

Promotor: J.M. Memelink

Promotores: Prof.dr. J. A. Bouwstra, Prof.dr. W. Jiskoot

Promotor: E. Gittenberger, Co-promotor: B.W. Hoeksema

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

The studies presented in the work show the potential of the integrative use of biophysical data in defining the structural basis of protein interactions.

Promotor: Prof.dr. J.P. Abrahams, Co-Promotor: N.S. Pannu

We describe certain birational modifications of nodal relative curves over a regular base of arbitrary dimension.

This report investigates the different approaches in the authorization process of GMOs for commercial use in the European Union and the United States of America. Three main aspects are considered and analyzed as potential causes of differences in the authorization process of GMOs for commercial use.…

We have examined sequences from the ligand-binding domain of the nicotinic acetyl choline receptor (nAChR) in 148 vertebrate species. We are in interested in this receptor because the α-neurotoxins of many venomous snakes binds to this receptor in its location at the neuromuscular junction in all ve…

Streptomyces are multicellular, Gram-positive bacteria in the phylum of actinobacteria which produce a high amount of bioactive natural products of which the expression is tightly coordinated with the life cycle.

The chemical and structural heterogeneity of toxoid vaccines makes their analysis challenging. However, detailed insights on a molecular level can be obtained by mass spectrometry.

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

Diacylglycerol lipases (DAGLα and DAGLβ) are responsible for the biosynthesis of the endocannabinoid 2-arachidonoylglycerol (2-AG) in the brain and peripheral tissues. Selective DAGLβ inhibitors have been proposed as a potential treatment for inflammatory diseases with reduced potential for central…

Leeuwen in West- en Centraal-Afrika vormen een unieke groep, die relatief weinig verwant is met leeuwen in Oost en zuidelijk Afrika. Biologen van de Universiteit Leiden bevestigen dit in een artikel gepubliceerd in Scientific Reports.

Promotor: Prof.dr. M. Danhof, Co-Promotor: J. de Jongh

Model based approaches, integrating physiological parameters or linking exposure with response, are powerful tools to quantify and evaluate the impact of genetic differences that are reflected as variability of drug exposure and/or clinical response(s). This thesis “Pharmacogenomics in Drug Development: …

Organisms often need to adapt more efficiently and devise new strategies for surviving difficult ecological circumstances.

Hybrid zones occur where two species meet and produce offspring (hybrids). Typically, hybrids show a considerable reduction in fitness. In this thesis two hybrid zones are treated.

Lysosomal storage disorders (LSDs) are a group of orphan diseases characterized by lysosomal dysfunction or impaired lysosomal catabolism and affect collectively about 1 in 5000 live births.

Promotores: Prof.dr. F. van Lunteren, Prof.dr. F.S. Gaastra

Promotie

This dissertation contains four works during my PhD. Different biomaterials have been designed based on coiled-coil peptides. These biomaterials have a range of applications, inclusing drug delivery, cell sorting to cell-cell fusion.

Promotor: G. A. van der Marel, Co-promotor: D. V. Filippov

An objective of this research was to achieve direct, well-defined and non-rate-limiting electron transfer between respiratory enzymes and the electrode surface by means of 'Q-wires'.

This Thesis describes the design, synthesis and evaluation as glycoprocessing enzyme inhibitors of focused libraries of iminosugars.

Nicotinamide adenine dinucleotide (NAD+) is the substrate used for the introduction of the ubiquitous and highly dynamic PTM in which either one or multiple adenosine diphosphate ribose (ADPr) moieties are covalently attached to a nucleophilic side chain of an specific amino acid in the target protein…