Ariadne Schmidt, Isabelle Devos and Julie de Groot provide you with refreshing insights concerning the study on urban singles in the period between 1200 and 1900.

On 14 December, Jouke Tegelaar defended the thesis 'Single supervision, single judicial protection? Towards effective judicial protection in Single Supervisory Mechanism composite procedures'. The doctoral research was supervised by Matthias Haentjens and Willemien den Ouden.

The single cell group (SCG) is part of the Metabolomics & Analytics Centre Our research is centered around developing innovating analytical methods tailored for small-volume, or single cell analysis to enable metabolomics-driven systems biology in personalized health strategies. We are always on…

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

Detecting nanoscopic objects plays an important role in nanoscience in particular, in the rapidly growing field of nanobiology. The forebear to modern super-resolution microscopy for single molecule investigation, is fluorescence microscopy.

Single-molecule spectroscopy has become a powerful method for using organic fluorescent molecules in numerous applications.

The sequence-dependence of biomolecular interactions involving nucleic acids and proteins is essential for numerous processes inside the cell. Insights into the underlying molecular mechanisms have been obtained using various biochemical and biophysical methods on two different levels — bulk and sin…

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

John van Noort

The directorate of Leiden Observatory consists of the Scientific Director, the Director of Education and the Director of Operations.

This thesis is a collection of experimental attempts to enhance photoluminescence of fluorescent molecules and quantum dots with single gold nanorods (GNRs) and relevant applications.

Since the early 1990s, one can isolate the optical signal of a single molecule and single-molecule spectroscopy has quickly grown into an important research field.

Mechanobiology is a major area in biology, however, mechanics is currently a missing element in drug development pipelines. This has led to a high cost for the society. Mechanotoxicity of drugs often appears as a long-term side effect of drugs, and has led to removal of drugs from the market. Furthermore,…

In this work, we investigate the minute circular dichroism effects of single nanoparticles.To this aim, we apply photothermal imaging with a polarization-modulated heating beam.

The goal is to reduce single use coffee cup use both at the university cafés and cofee machines.

Promotores: Prof.dr. D. Bouwmeester, Prof.dr. A. Fiore (TU Eindhoven)

An Ancient Egyptian System of Workmen’s Identity Marks

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

Visualization of biomolecules in living cells by tagging with gold nanoparticles.

Spectroscopic studies on fluorescent single molecules in organic condensed matter does not only provide information about the molecule itself, but also its near environment. By suppression of phonon-induced broadening of spectral lines through cooling to low temperatures, small changes in the spectral…

We study cell-fate decision-making using embryonic stem cells as a model system. Stem cells integrate a large number of cues to direct their development into a great variety of cell types.

Promotor: Prof.dr. T. Schmidt, Co-promotor: Dr. ir. S. J.T. van Noort

Curved single crystals provide variable, but well-defined surface structures.

Promotor: Prof.dr. M.A.G.J. Orrit

Promotores: Prof.dr. G.W. Canters, Prof.dr. T.J. Aartsma

Do you want to be part of this unique programme, discuss cutting-edge topics of cyber security with other professionals, but do you not have time to enroll in this full master programme?

Promotor: Prof.dr. T. Hankemeier, Co-promotores: Heiko van der Linden, Paul Vulto

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

The use-wear analysis of domestic implements provided new insights of the Single Grave Culture population in the Netherlands.

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

This thesis aims to improve the detection from ultra-weak single emitter by enhancing their emission properties with plasmonic nanostructures. We exploit the wet-chemically synthesized single crystalline gold nanorods (GNRs) as our basic frameworks in the whole studies, simply because of their unique…

A single self-assembled semiconductor quantum dot in a high-finesse optical microcavity - the subject of this thesis - is an interesting quantum-mechanical system for future quantum applications. For instance, this system allows trapping of an extra electron and thus can serve as a spin quantum memory,…

Gold nanorods are biocompatible nanoparticles that present an excellent two-photon signal that can be used to get high spatial resolution inside living cells. Gold nanorods are photostable and therefore can be followed inside cells for long time, with possible applications as trackers in live cells.

Early time-resolved experiments by Frauenfelder on the ensemble of the kinetic rebinding of CO to myoglobin molecules resulted in a stretched exponential relaxa-tion due to a very large spread of the reaction rates of individual molecules.

Promotor: M.A.G.J. Orrit

Science, Leiden Institute of Physics (LION)

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Microneedles represent promising tools for delivery of drugs to the skin. However, before these microneedles can be used in clinical practice, it is essential to understand the process of skin penetration by these microneedles.

Can we find new chemical and biological sensing routes on the edge and surface of graphene to improve the potential of graphene to act as a sensor?

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

The deeper understanding of an organism's pathology is important for developing treatments. Over centuries of systematic research, clinical researchers have demonstrated that the more information they acquire about the cellular properties and their organisation in the tissue, the better they can understand…

Optical detection of a single electron using a single molecule has never been done. Leiden physicist Michel Orrit and his team have now identified a molecule that is sensitive enough to detect an electron at a distance of hundreds of nanometers. The results are published as a cover article in ChemPh…

This thesis consists of two main parts. The first part (Chapters 2 and 3) deals with a class of Markov process called Quasi-Skipfree (QSF) processes.

Metal-based prodrugs based on ruthenium(II) polypyridyl complexes have proven to be very suitable for application in both photodynamic therapy (PDT) and photo-activated chemotherapy (PACT).

A lecturer winning a prize, an expert appearing in the media or a professor giving their inaugural speech: these are faces we often see. But we rarely see the staff who work behind the scenes to make their work possible. They are now featured in the photo exhibition ‘The forces behind the faces’.

Promotores: Prof.dr. H.J.M. de Groot, Prof.dr. J. Matysik (Universitaet Leipzig)

The focus throughout this thesis will be on gathering fundamental studies of the detailed structure and composition of the electrode/electrolyte interface effect on the rate and mechanism of key electrocatalytic reactions.

The “harpe organisée”, 1720-1840: Rediscovering the lost pedal techniques on harps with a single-action pedal mechanism, is the title of Maria Christina Cleary's PhD thesis. This is the first monographic study on harp pedal techniques, tracing the historical way to pedal on the early pedal harps with…

Leiden physicists have developed a way to address how accurately a superconducting single photon detector (SSPD) can be characterized by detector tomography. SSPDs are not fully understood, and tomography is a key element to determine how these devices detect light. A better understanding of these detectors…